Determination of Nobiletin in Rat Plasma after Ingestion of <i>Citrus depressa</i> Juice by Capillary Liquid Chromatography with Electrochemical Detection Using Boron-doped Diamond Electrode
At a Glance
Section titled āAt a Glanceā| Metadata | Details |
|---|---|
| Publication Date | 2015-01-01 |
| Journal | Electrochemistry |
| Authors | A. Kotani, Kota NAGAMI, Chika MINO, Yasuhito SUGAWARA, Kouji Takahashi |
| Institutions | Tokyo University of Pharmacy and Life Sciences |
| Citations | 9 |
Abstract
Section titled āAbstractāVoltammetric detection using a boron doped diamond (BDD) electrode coupled with capillary liquid chromatography (CLC) was developed for determining nobiletin, one of the polymethoxyflavones (PMFs), in plasma samples. CLC with electrochemical detection (CLC-ECD) was performed using a capillary octadecylsilica (ODS) column, water-acetonitrile-phosphoric acid (65:35:0.5, v/v/v), as a mobile phase, and an applied potential at +1.45 V vs. Ag/AgCl. Chromatographic peak heights were found linearly related to the amounts of nobiletin injected from 0.48 pg to 0.40 ng (r = 0.999). The detection limit (S/N = 3) was 0.13 pg. The concentrations of nobiletin in rat plasma after oral ingestion of 5.0 mL kgā1 Citrus depressa juice, corresponding to 0.17 mg kgā1 nobiletin, were determined to obtain a concentration-time profile of nobiletin by the present CLC-ECD method, which required only 30 ĀµL of plasma sample for each time point. Based on the concentration-time profile of nobiletin in the rat plasma, the pharmacokinetic parameters of maximum drug concentration (Cmax), maximum drug concentration time (tmax), and area under the concentration-time curve (AUC0-3 h) were obtained.