SIMULTANEOUS VOLTAMMETRIC DETERMINATION OF AMLODIPINE BESYLATE AND HYDROCHLOROTHIAZIDE IN SYNTHETIC URINE SAMPLES USING A BORON-DOPED DIAMOND ELECTRODE
At a Glance
Section titled āAt a Glanceā| Metadata | Details |
|---|---|
| Publication Date | 2015-01-01 |
| Journal | QuĆmica Nova |
| Authors | LaĆs Pereira Silva, Bruna C. LourenĆ§Ć£o, Orlando FatibelloāFilho |
| Institutions | Universidade Federal de SĆ£o Carlos |
| Citations | 24 |
Abstract
Section titled āAbstractāA novel method for simultaneous voltammetric determination of the antihypertensive drugs, amlodipine besylate (AML) and hydrochlorothiazide (HTZ) in synthetic urine samples was developed. Determination was carried out with a cathodically pretreated boron-doped diamond electrode (CPT-BDD) using square-wave voltammetry (SWV) and Britton-Robinson buffer solution at pH 2 as the supporting electrolyte. AML and HTZ exhibited oxidation peaks at 0.88 V and 1.30 V, respectively. The difference in potentials of 0.42 V allowed the simultaneous determination of these two analytes. The analytical curve showed linear response in the concentration range from 0.200 to 9.09 Āµmol L<sup>-1</sup> for AML and from 4.00 to 100 Āµmol L<sup>-1</sup> for HTZ, with a limit of detection (LOD) of 60.0 nmol L<sup>-1</sup> and of 2.00 Āµmol L<sup>-1</sup> for AML and HTZ, respectively. Recoveries of these analytes ranged from 91.0 to 107%. The proposed method using SWV and a CPT-BDD electrode is simple, fast and low cost, representing a good alternative for simultaneous determination of AML and HTZ in urine samples.