Skip to content
6CCVD Research

Antimicrobial activity of different biological extracts as intracanal medicament against Enterococcus faecalis - An in vitro study

At a Glance

Section titled ā€œAt a Glanceā€
MetadataDetails
Publication Date2016-01-01
JournalEndodontology
AuthorsMuktishree Mahendra, Nikita Agrawal, Swapna Munaga, Sanjeev Tyagi
Citations10

Abstract

Section titled ā€œAbstractā€

Introduction: A successful endodontic treatment depends upon complete debridement of microflora from the root canal system. However, due to complex root canal configuration, complete debridement through mechanical instrumentation alone cannot remove entire bacterial load. So the aim is in vitro evaluation of antimicrobial efficacy of biological extracts against Enterococcus faecalis MTCC-439 strain when used as intracanal medicaments. The medicaments used were Nissin, an antibiotic peptide; neem known for its antiseptic properties; platelet rich plasma (PRP) known for its regenerative properties and propolis, a resin extract derived from bees. Materials and Methods: Sixty single rooted lower premolar teeth which were extracted for orthodontic purpose were collected. Tooth specimens were sectioned at cement-enamel junction with a diamond saw to obtain a standard root length. The root canals of the specimen were instrumented with K3 rotary files followed by inoculation of E. faecalis strains and sealed with dental wax. The specimens were then kept in incubator for 21 days at 37Ā°C and after that randomly divided into six treatment groups: Group I, 5 Ī¼L Normal saline; Group II, 5 Ī¼L Nisin (Vasta Biotech, Chennai); Group III, 5 Ī¼L propolis; Group IV, 5 Ī¼L neem; Group V, 5 Ī¼L PRP; Group VI, 5 Ī¼L Calcium hydroxide. Roots were then incubated for 7 days at 37Ā°C. On 8th day, to evaluate the degree of infection, dentin chips from root canal of specimens were extracted with a sterile 6% K3 rotary file. Kruskal-Wallis test and Mann-Whitney U-test were applied for difference in colony forming units (CFUs) count for different medicaments. Results: In present in vitro study, Nisin showed no CFU while neem and propolis showed significantly less growth as compared to PRP and calcium hydroxide against E. faecalis. Conclusions: Nisin outreach propolis and neem in eliminating the E. faecalis when used as intracanal medicaments.

Tech Support

Section titled ā€œTech Supportā€

Original Source

Section titled ā€œOriginal Sourceā€

References

Section titled ā€œReferencesā€
  1. 1982 - Bacterial invasion of pulpal dentin wall in vitro
  2. 2003 - Effectiveness of 2% chlorhexidine gel and calcium hydroxide against Enterococcus faecalis in bovine root dentine in vitro
  3. 2008 - Evaluation of Morinda citrifolia as an endodontic irrigant
  4. 2012 - Effectiveness of endodontic disinfecting solutions against young and old Enterococcus faecalis biofilms in dentin canals
  5. 2001 - Aetiology of root canal treatment failure: why well-treated teeth can fail
  6. 1987 - In vitro infection and disinfection of dentinal tubules
  7. 2003 - Efficacy of calcium hydroxide: chlorhexidine paste as an intracanal medication in bovine dentin
  8. 1999 - , Lopes HP.Mechanisms of antimicrobial activity of calcium hydroxide: a critical review
  9. 1998 - Antibacterial efficacy of nisin against multidrug-resistant Gram-positive pathogens

Reads Similar to This

Amination of Boron-Doped Diamond Surfaces Nanocarbon Composites for Non-Incandescent Microwave Magnetrons and Subterahertz Ranges Effect of different non-invasive and micro-invasive treatment modalities of white spot lesions on microhardness of human enamel