Preparation of hemoglobin-modified boron-doped diamond for acrylamide biosensors
At a Glance
Section titled āAt a Glanceā| Metadata | Details |
|---|---|
| Publication Date | 2017-04-01 |
| Journal | IOP Conference Series Materials Science and Engineering |
| Authors | Khoirul Umam, Endang Saepudin, Tribidasari A. Ivandini |
| Institutions | University of Indonesia |
| Citations | 10 |
Abstract
Section titled āAbstractāBoron-doped diamond (BDD) electrode was modified with haemoglobin to develop electrochemical biosensors of acrylamide. Prior to modify with haemoglobin, the BDD was modified by gold nanoparticles to increase the affinity of BDD against haemoglobin. The electrochemical behaviour of the electrode in the presence of acrylamide was studied in comparison to haemoglobin-modified gold electrodes. Cyclic voltammetry indicated the optimum responses in 0.1 M sodium acetate buffer at pH 5. The responses were linear to the acrylamide concentration range of 5-50 Ī¼M with an estimated detection limit of 5.14 Ī¼M, suggesting that the electrode was promising for acrylamide biosensors.