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6CCVD Research

Aptamer-Based Carboxyl-Terminated Nanocrystalline Diamond Sensing Arrays for Adenosine Triphosphate Detection

At a Glance

Section titled ā€œAt a Glanceā€
MetadataDetails
Publication Date2017-07-21
JournalSensors
AuthorsEvi Suaebah, Takuro Naramura, Miho Myodo, Masataka Hasegawa, Shuichi Shoji
InstitutionsWaseda University, Photonics Electronics Technology Research Association
Citations8

Abstract

Section titled ā€œAbstractā€

Here, we propose simple diamond functionalization by carboxyl termination for adenosine triphosphate (ATP) detection by an aptamer. The high-sensitivity label-free aptamer sensor for ATP detection was fabricated on nanocrystalline diamond (NCD). Carboxyl termination of the NCD surface by vacuum ultraviolet excimer laser and fluorine termination of the background region as a passivated layer were investigated by X-ray photoelectron spectroscopy. Single strand DNA (amide modification) was used as the supporting biomolecule to immobilize into the diamond surface via carboxyl termination and become a double strand with aptamer. ATP detection by aptamer was observed as a 66% fluorescence signal intensity decrease of the hybridization intensity signal. The sensor operation was also investigated by the field-effect characteristics. The shift of the drain current-drain voltage characteristics was used as the indicator for detection of ATP. From the field-effect characteristics, the shift of the drain current-drain voltage was observed in the negative direction. The negative charge direction shows that the aptamer is capable of detecting ATP. The ability of the sensor to detect ATP was investigated by fabricating a field-effect transistor on the modified NCD surface.

Tech Support

Section titled ā€œTech Supportā€

Original Source

Section titled ā€œOriginal Sourceā€

References

Section titled ā€œReferencesā€
  1. 2005 - FluMag-SELEX as an advantageous method for DNA aptamer selection [Crossref]
  2. 2011 - Aptamer based Fluorescent Biosensor [Crossref]
  3. 2013 - Nucleic acid aptamers as high affinity ligands in biotechnology and biosensorics
  4. 2011 - Aptamer modules as sensors and detectors [Crossref]
  5. 2014 - Aptamer-based biosensors for biomedical diagnostics [Crossref]
  6. 2008 - ATP detection using a label-free DNA aptamer and a cationic tetrahedralfluorene [Crossref]
  7. 2011 - Diamond electrolyte solution gate FETs for DNA and protein sensors using DNA/RNA aptamers [Crossref]
  8. 2017 - Diamond nanostructures for drug delivery, bioimaging, and biosensing [Crossref]
  9. 2007 - Diamond for bio-sensor applications [Crossref]

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