A microsensing system for the <i>in vivo</i> real-time monitoring of local drug kinetics
At a Glance
Section titled āAt a Glanceā| Metadata | Details |
|---|---|
| Publication Date | 2018-01-01 |
| Journal | Proceedings for Annual Meeting of The Japanese Pharmacological Society |
| Authors | Genki Ogata, Kai Asai, Yamato Sano, Madoka Takai, Hiroyuki Kusuhara |
| Institutions | Keio University, The University of Tokyo |
Abstract
Section titled āAbstractāIt is crucial to continuously measure local concentrations of systemically administered drugs in vivo in different organs and tissues. However, conventional methods require considerable samples quantities and have poor sampling rates. Additionally, they cannot address how drug kinetics correlates with target function over time. In this study, we developed a system with two different sensors. One is a needle-type of boron-doped diamond microsensor with tip diameter ļ½40 Āµm. This sensor can detect change of drug concentrations with time resolution of ļ½5 seconds. The other is a glass microelectrode for monitoring cellular electrophysiological responses. We first tested the bumetanide, a blocker for Na+,K+,2Clācotransporter blocker. This diuretic can induce deafness. In the guinea-pig cochlea injected intravenously with bumetanide, the changes of the drug concentration and the extracellular potential underlying hearing were simultaneously measured in real time. We further examined an antiepileptic drug lamotrigine, which inhibits Na+ channel, in the rat brain, and tracked its kinetics and at the same time the local field potentials mirroring neuronal activity. The action of the anticancer reagent doxorubicin was also monitored in vivo. This microsensing system may be applied to analyze pharmacokinetics and pharmacodynamics of various drugs at local sites.