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6CCVD Research

Electrochemical Sensor Based on Molecularly Imprinted Polymer for the Detection of Cefalexin

At a Glance

Section titled “At a Glance”
MetadataDetails
Publication Date2019-02-27
JournalBiosensors
AuthorsBogdan Feier, Adrian Blidar, Alexandra Pusta, Paula Carciuc, CĂ©cilia Cristea
InstitutionsIuliu Hațieganu University of Medicine and Pharmacy
Citations56

Abstract

Section titled “Abstract”

In this study, a new electrochemical sensor was developed for the detection of cefalexin (CFX), based on the use of a molecularly imprinted polymer (MIP) obtained by electro‒polymerization in an aqueous medium of indole-3-acetic acid (I3AA) on a glassy carbon electrode (GCE) and on boron-doped diamond electrode (BDDE). The two different electrodes were used in order to assess how their structural differences and the difference in the potential applied during electrogeneration of the MIP translate to the performances of the MIP sensor. The quantification of CFX was performed by using the electrochemical signal of a redox probe before and after the rebinding of the template. The modified electrode was characterized using atomic force microscopy (AFM), scanning electron microscopy (SEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The influence of different parameters on the fabrication of the sensor was tested, and the optimized method presented high selectivity and sensitivity. The MIP-based electrode presented a linear response for CFX concentration range of 10 to 1000 nM, and a limit of detection of 3.2 nM and 4.9 nM was obtained for the BDDE and the GCE, respectively. The activity of the sensor was successfully tested in the presence of some other cephalosporins and of other pharmaceutical compounds. The developed method was successfully applied to the detection of cefalexin from real environmental and pharmaceutical samples.

Tech Support

Section titled “Tech Support”

Original Source

Section titled “Original Source”

References

Section titled “References”
  1. 2012 - Novel microbiological system for antibiotic detection in ovine milk [Crossref]
  2. 2007 - Quantification of cephalexin as residue levels in bovine milk by high-performance liquid chromatography with on-line sample cleanup [Crossref]
  3. 2016 - Multiresidue analysis of cephalosporin antibiotics in bovine milk based on molecularly imprinted polymer extraction followed by liquid chromatography-tandem mass spectrometry [Crossref]
  4. 2016 - Simultaneous determination of 22 cephalosporins drug residues in pork muscle using liquid chromatography-tandem mass spectrometry [Crossref]
  5. 2015 - Synthesis and application of cephalexin imprinted polymer for solid phase extraction in milk [Crossref]
  6. 2015 - New spectrophotometric method for determination of cephalosporins in pharmaceutical formulations [Crossref]

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