Electrochemical Determination of Vitamin D 3 in Pharmaceutical Products by Using Boron Doped Diamond Electrode
At a Glance
Section titled āAt a Glanceā| Metadata | Details |
|---|---|
| Publication Date | 2019-11-12 |
| Journal | Electroanalysis |
| Authors | Ana ÄuroviÄ, Zorica StojanoviÄ, Snežana KraviÄ, Jovana Kos, LukĆ”Å” Richtera |
| Institutions | Brno University of Technology, Central European Institute of Technology |
| Citations | 28 |
Abstract
Section titled āAbstractāAbstract A sensitive squareāwave voltammetry method was developed to determine cholecalciferol (vitamin D 3 ) in pharmaceutical products at boronādoped diamond electrode as a working electrode. Vitamin D 3 provided a wellādefined voltammetric peak at around +1.00 V ( vs . Ag/AgCl, 3.5 mol dm ā3 ) in 0.02 mol dm ā3 BrittonāRobinson buffer pH 5.0 prepared in 50 % ethanol. The influence of various factors such as type and pH of the supporting electrolyte, scan rate and squareāwave parameters were studied and optimized. Under optimum conditions, the oxidation peak current increased linearly with the concentration of vitamin D 3 over the range of 2 to 200 Ī¼mol dm ā3 . The calculated limit of detection and limit of quantitation were 0.17 Ī¼mol dm ā3 and 0.51 Ī¼mol dm ā3 , respectively. The boronādoped diamond electrode exhibited specific recognition capability for cholecalciferol amongst possible interferences, and the determination of vitamin D 3 was possible in samples such as commercial pharmaceutical products without complicated sample pretreatments.
Tech Support
Section titled āTech SupportāOriginal Source
Section titled āOriginal SourceāReferences
Section titled āReferencesā- 2017 - The Vitamins Fundamental Aspects in Nutrition and Health, 5th