Electrochemical Determination of the Steroid Tibolone and Its Metabolites in Saliva Samples
At a Glance
Section titled āAt a Glanceā| Metadata | Details |
|---|---|
| Publication Date | 2020-11-02 |
| Journal | ChemElectroChem |
| Authors | Weberson P. Silva, Raquel G. Rocha, John Kenedy Rodrigues Pereira Felisbino, Raquel Sousa, Rodrigo A.A. MuƱoz |
| Institutions | Universidade Federal de UberlĆ¢ndia |
| Citations | 5 |
Abstract
Section titled āAbstractāAbstract Tibolone (TIB) is a synthetic steroid banned by the World AntiāDoping Agency (WADA) due to its similar properties to estrogen and progesterone. In this work, a simple and lowācost electrochemical method for the determination of TIB and its metabolites (3āĪ± hydroxytibolone and 3āĪ² hydroxytibolone) in authentic human saliva is reported. Squareāwave voltammetry (SWV) with an unmodified boronādoped diamond (BDD) electrode and phosphate buffer 0.1 mol L ā1 were selected as the electrochemical technique, working electrode, and supporting electrolyte, respectively. Analytical characteristics of the proposed method include good repeatability (RSD <2.4 %), wide linear range (1 to 110 Ī¼mol L ā1 ; r=0.999) and low detection limit (0.3 Ī¼mol L ā1 ). Average recovery values (104Ā±3 % for TIB and 98Ā±5 % for the metabolites) for direct analysis of spiked saliva samples (only diluted in supporting electrolyte) indicate that no significant matrix interference was observed. The proposed method was also efficient for the determination of TIB in pharmaceutical samples (statistically similar results to those obtained by HPLC).