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6CCVD Research

Highly Sensitive Determination of Atropine in Pharmaceuticals, Biological Fluids and Beverage on Planar Electrochemical Cell with Working Boron-Doped Diamond Electrode

At a Glance

Section titled ā€œAt a Glanceā€
MetadataDetails
Publication Date2022-09-10
JournalElectrochimica Acta
AuthorsOlha Dushna, Liliya Dubenska, MariƔn Vojs, MariƔn Marton, Igor Patsay
InstitutionsSlovak University of Technology in Bratislava, SCIVP of Veterinary Medicinal Products and Feed Additives
Citations29

Abstract

Section titled ā€œAbstractā€

In the present paper, for the first time, a planar electrochemical cell formed of a novel screen-printed sensor with the working boron-doped diamond electrode (BDDE) was used for the voltammetric determination of alkaloid atropine (ATR). Different BDDE diameters of 1, 2 and 3 mm were used. ATR gives one irreversible oxidation peak at the potential of 1.5 V in the presence of 2 M HClO4. Two irreversible peaks of ATR oxidation at potentials 1.0 V and 1.2 V were obtained using Britton-Robinson buffer at pH 11. Differential pulse voltammetry (DPV) and square-wave voltammetry (SWV) were used to obtain calibration curves for ATR on BDDE. The use of cells with various BDDE diameters enables an expansion of the range of linear concentration. The limit of detection (LOD) reaches the lowest value of 0.08 Ī¼Šœ using DPV on BDDE with the diameter of 3 mm. Using the working electrode with the diameter of 1 mm it is possible to determine 4.1 Ī¼Šœ ATR. ATR can be determined in drugs (without multistage dilution and separation) and biological fluids using planar cells. The method has also been approved to determine ATR at the lethal dose level and below in Cola. One drop of the beverage is enough for this analysis. The developed methods were validated according to the criteria such as linearity, limits of detection and quantification, accuracy, precision, stability, and selectivity. The presented analysis protocols are rapid, inexpensive, portable, and relevant.

Tech Support

Section titled ā€œTech Supportā€

Original Source

Section titled ā€œOriginal Sourceā€

References

Section titled ā€œReferencesā€
  1. 2012 - A simple and sensitive GC/MS method for the determination of atropine during therapy of anticholinesterase poisoning in serum samples [Crossref]
  2. 2019 - Utilization of an electrochemiluminescence sensor for atropine determination in complex matrices [Crossref]
  3. 2011 - A case of acute iatrogenic atropine poisoning [Crossref]
  4. 2012 - Atropa belladonna intoxication: a case report
  5. 2006 - Quantification by HPLC-MS/MS of atropine in human serum and clinical presentation of six mildto-moderate intoxicated atropine-adulterated-cocaine users [Crossref]
  6. 2013 - Scientific opinion on tropane alkaloids in food and feed

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