Determination of Coumarin in Mikania glomerata Infusions by Square-Wave Voltammetry Using a Boron-Doped Diamond Electrode
At a Glance
Section titled āAt a Glanceā| Metadata | Details |
|---|---|
| Publication Date | 2022-12-09 |
| Journal | Journal of the Brazilian Chemical Society |
| Authors | Denise M. NĆ³brega, Anabel S. LourenƧo, David Fernandes, Wellington da Silva Lyra, Edilene D.T. Moreira |
| Citations | 1 |
Abstract
Section titled āAbstractāInfusions of Mikania glomerata Sprengel (guaco) leaves are used in Brazil for the treatment of respiratory diseases. Coumarin is the chemical marker of guaco for quality control purposes. This work proposes a voltammetric methodology to determine coumarin by using a borondoped diamond electrode. At pH 10 (0.1 mol L-1 Britton-Robinson buffer solution), coumarin is irreversibly oxidized around +1.77 V in a process predominantly controlled by diffusion. The methodology described here presented a linear range from 1.54 to 15.3 Ī¼g mL-1 with limits of detection and quantification of 0.20 and 0.62 Ī¼g mL-1, respectively. Analysis of variance confirmed the significance of the regression and the absence of lack-of-fit at the confidence level of 95%. Infusion of commercial guaco leaves was directly analyzed, and results were in agreement with high-performance liquid chromatography after applying the paired t-test at a confidence level of 95%.