Quantitative Evaluation of the Cellular Uptake of Nanodiamonds by Monocytes and Macrophages
At a Glance
Section titled âAt a Glanceâ| Metadata | Details |
|---|---|
| Publication Date | 2023-01-13 |
| Journal | Small |
| Authors | ÎαÏία ÎÎčÏÏα, Mathilde H. Lerche, Martin Dufva, Kirstine BergâSĂžrensen |
| Institutions | Ărsted (Denmark), Technical University of Denmark |
| Citations | 9 |
Abstract
Section titled âAbstractâAbstract Fluorescent nanodiamonds (FNDs) with negative nitrogenâvacancy (NV â ) defect centers are great probes for biosensing applications, with potential to act as biomarkers for cell differentiation. To explore this concept, uptake of FNDs (â120 nm) by THPâ1 monocytes and monocyteâderived M0âmacrophages is studied. The time course analysis of FND uptake by monocytes confirms differing FNDâcell interactions and a positive timeâdependence. No effect on cell viability, proliferation, and differentiation potential into macrophages is observed, while cells saturated with FNDs, unload the FNDs completely by 25 cell divisions and subsequently take up a second dose effectively. FND uptake variations by THPâ1 cells at early exposureâtimes indicate differing phagocytic capability. The cell fraction that exhibits relatively enhanced FND uptake is associated to a macrophage phenotype which derives from spontaneous monocyte differentiation. In accordance, chemicalâdifferentiation of the THPâ1 cells into M0âmacrophages triggers increased and homogeneous FND uptake, depleting the fraction of cells that were nonâresponsive to FNDs. These observations imply that FND uptake allows for distinction between the two cell subtypes based on phagocytic capacity. Overall, FNDs demonstrate effective cell labeling of monocytes and macrophages, and are promising candidates for sensing biological processes that involve cell differentiation.