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6CCVD Research

Evaluation of In Vitro Serotonin-Induced Electrochemical Fouling Performance of Boron Doped Diamond Microelectrode Using Fast-Scan Cyclic Voltammetry

At a Glance

Section titled ā€œAt a Glanceā€
MetadataDetails
Publication Date2024-07-19
JournalBiosensors
AuthorsMason L. Perillo, Bhavna Gupta, James R. Siegenthaler, Isabelle E. Christensen, Brandon Kepros
InstitutionsMichigan State University, Fraunhofer USA
Citations4

Abstract

Section titled ā€œAbstractā€

Fast-scan cyclic voltammetry (FSCV) is an electrochemical sensing technique that can be used for neurochemical sensing with high spatiotemporal resolution. Carbon fiber microelectrodes (CFMEs) are traditionally used as FSCV sensors. However, CFMEs are prone to electrochemical fouling caused by oxidative byproducts of repeated serotonin (5-HT) exposure, which makes them less suitable as chronic 5-HT sensors. Our team is developing a boron-doped diamond microelectrode (BDDME) that has previously been shown to be relatively resistant to fouling caused by protein adsorption (biofouling). We sought to determine if this BDDME exhibits resistance to electrochemical fouling, which we explored on electrodes fabricated with either femtosecond laser cutting or physical cleaving. We recorded the oxidation current response after 25 repeated injections of 5-HT in a flow-injection cell and compared the current drop from the first with the last injection. The 5-HT responses were compared with dopamine (DA), a neurochemical that is known to produce minimal fouling oxidative byproducts and has a stable repeated response. Physical cleaving of the BDDME yielded a reduction in fouling due to 5-HT compared with the CFME and the femtosecond laser cut BDDME. However, the femtosecond laser cut BDDME exhibited a large increase in sensitivity over the cleaved BDDME. An extended stability analysis was conducted for all device types following 5-HT fouling tests. This analysis demonstrated an improvement in the long-term stability of boron-doped diamond over CFMEs, as well as a diminishing sensitivity of the laser-cut BDDME over time. This work reports the electrochemical fouling performance of the BDDME when it is repeatedly exposed to DA or 5-HT, which informs the development of a chronic, diamond-based electrochemical sensor for long-term neurotransmitter measurements in vivo.

Tech Support

Section titled ā€œTech Supportā€

Original Source

Section titled ā€œOriginal Sourceā€

References

Section titled ā€œReferencesā€
  1. 2005 - Neurotransmitters [Crossref]
  2. 2016 - Dysregulation of the dopamine system in the pathophysiology of schizophrenia and depression [Crossref]
  3. 2017 - Reward Circuitry in Addiction [Crossref]
  4. 2018 - Beyond the dopamine hypothesis of schizophrenia to three neural networks of psychosis: Dopamine, serotonin, and glutamate [Crossref]
  5. 2016 - Subsecond dopamine fluctuations in human striatum encode superposed error signals about actual and counterfactual reward [Crossref]
  6. 2016 - Cocaine Self-Administration Experience Induces Pathological Phasic Accumbens Dopamine Signals and Abnormal Incentive Behaviors in Drug-Abstinent Rats [Crossref]
  7. 2010 - In vivo voltammetric monitoring of catecholamine release in subterritories of the nucleus accumbens shell [Crossref]
  8. 2024 - Serotonin as a biomarker of toxin-induced Parkinsonism [Crossref]
  9. 2020 - Fundamentals of fast-scan cyclic voltammetry for dopamine detection [Crossref]

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