What Are the Key Factors for the Detection of Peptides Using Mass Spectrometry on Boron-Doped Diamond Surfaces?
At a Glance
Section titled āAt a Glanceā| Metadata | Details |
|---|---|
| Publication Date | 2024-07-24 |
| Journal | Nanomaterials |
| Authors | Juvissan Aguedo, MariĆ”n Vojs, Martin VrÅ”ka, Marek NemÄoviÄ, Zuzana PakanovĆ” |
| Institutions | Institute of Chemistry of the Slovak Academy of Sciences, Czech Academy of Sciences, Institute of Physics |
Abstract
Section titled āAbstractāWe investigated the use of boron-doped diamond (BDD) with different surface morphologies for the enhanced detection of nine different peptides by matrix-assisted laser desorption/ionisation mass spectrometry (MALDI-MS). For the first time, we compared three different nanostructured BDD film morphologies (Continuous, Nanograss, and Nanotips) with differently terminated surfaces (-H, -O, and -F) to commercially available Ground Steel plates. All these surfaces were evaluated for their effectiveness in detecting the nine different peptides by MALDI-MS. Our results demonstrated that certain nanostructured BDD surfaces exhibited superior performance for the detection of especially hydrophobic peptides (e.g., bradykinin 1-7, substance P, and the renin substrate), with a limit of detection of down to 2.3 pM. Further investigation showed that hydrophobic peptides (e.g., bradykinin 1-7, substance P, and the renin substrate) were effectively detected on hydrogen-terminated BDD surfaces. On the other hand, the highly acidic negatively charged peptide adrenocorticotropic hormone fragment 18-39 was effectively identified on oxygen-/fluorine-terminated BDD surfaces. Furthermore, BDD surfaces reduced sodium adduct contamination significantly.
Tech Support
Section titled āTech SupportāOriginal Source
Section titled āOriginal SourceāReferences
Section titled āReferencesā- 2023 - Development of nanomaterial enabling highly sensitive surface-assisted laser desorption/ionization mass spectrometry peptide analysis [Crossref]
- 2022 - MALDI-TOF Mass Spectrometry in Clinical Analysis and Research [Crossref]
- 2023 - Identification of Conformational Variants for Bradykinin Biomarker Peptides from a Biofluid Using a Nanopore and Machine Learning [Crossref]
- 1999 - Desorption-ionization mass spectrometry on porous silicon [Crossref]
- 2000 - An activated carbon substrate surface for laser desorption mass spectrometry [Crossref]
- 2018 - Multifunctional Au NPs-polydopamine-polyvinylidene fluoride membrane chips as probe for enrichment and rapid detection of organic contaminants [Crossref]
- 2019 - Ag nanoparticles/ZnO nanorods for highly sensitive detection of small molecules with laser desorption/ionization mass spectrometry [Crossref]
- 2019 - Magnetic metal-organic framework nanocomposites for enrichment and direct detection of environmental pollutants by negative-ion matrix-assisted laser desorption/ionization time-of-flight mass spectrometry [Crossref]