Geobacter sulfurreducens Immobilized Boron-Doped Diamond Electrodes for Uranium(VI) in Water Electrochemical Bioremediation
At a Glance
Section titled âAt a Glanceâ| Metadata | Details |
|---|---|
| Publication Date | 2025-09-14 |
| Journal | ACS Omega |
| Authors | Alexis J. Acevedo-GonzĂĄlez, Elliott G. Rosario-Cruz, Angelica A. Chacon, Gary A. Toranzos, Carlos R. Cabrera |
| Institutions | University of Puerto Rico at RĂo Piedras, The University of Texas at El Paso |
Abstract
Section titled âAbstractâThe proliferation of nuclear science and technology has resulted in an increase in nuclear waste containing uranium, posing significant risks to both human and environmental health. This study proposes the use of <i>Geobacter sulfurreducens</i> (<i>G. sulfurreducens</i>) modified boron-doped diamond electrodes to facilitate the reduction and removal of uranium-(VI) from aqueous media. The bioremediation process involves electrochemically immobilizing the bacteria on a boron-doped diamond electrode (BDD). The immobilization process requires applying reduction potentials ranging from -0.40 to -0.70 V (vs Ag/AgCl (3 M NaCl)), with -0.60 V identified as the optimal potential for effective bacterial modification. The uranium source is provided by a 2.0 mM uranyl acetate solution in <i>G. sulfurreducens</i> growth medium. Scanning electron microscopy (SEM) reveals a highly uniform layer of uranium on the electrode surface. Energy-dispersive X-ray fluorescence spectroscopy (EDS) and cyclic voltammetry (CV) studies confirm the presence of uranium in the system. Raman spectroscopy and X-ray photoelectron spectroscopy (XPS) successfully elucidate the reduction process of U-(VI) to predominantly U-(IV) using a bacteria-electrode coupled system. Additionally, a comparison is made with the electrochemical removal of uranyl ions using the electrodeposition method on unmodified BDD. Results demonstrate the presence of three uranium oxide species (UO<sub>2</sub>, UO<sub>3</sub>, and U<sub>3</sub>O<sub>8</sub>) on the BDD electrode after experimentation, in contrast to the <i>G. sulfurreducens</i>/BDD assembly, which achieves the predominant reduction of UO<sub>2</sub> <sup>2+</sup> to UO<sub>2</sub> with a small quantity of UO<sub>3</sub> as the final species. This study highlights the efficient electrochemical removal of uranyl ions from aqueous media at the <i>G. sulfurreducens</i>/BDD interface through chronoamperometry, presenting a promising approach for remediating sites contaminated with radioactive materials. The findings contribute to the exploration of sustainable alternatives for managing nuclear waste, emphasizing the potential of this electrochemical bioremediation strategy.