A Silver‐Stain Modification of Standard Histological Slide Preparation for Use in Anthropology Analyses
At a Glance
Section titled “At a Glance”| Metadata | Details |
|---|---|
| Publication Date | 2015-01-12 |
| Journal | Journal of Forensic Sciences |
| Authors | Deborrah C. Pinto, Eric D. Pace |
| Institutions | Institute of Forensic Sciences |
| Citations | 7 |
Abstract
Section titled “Abstract”Abstract The traditional histology method typically employed by forensic anthropologists involves plastic embedding of undecalcified bone. The embedded sample is then cut by a diamond blade saw and ground to the required thickness of ~50-100 microns using a grinder. There are several limitations to this method: Cement lines may be blurred; depth‐of‐field artifacts may result from viewing thick sections; and medicolegal offices with limited budgets may not be able to invest in additional equipment or training for this method. A silver nitrate stain modification of the standard histology preparation technique of decalcified bone is presented. The benefits of this technique are that: Cement lines are viewed clearly; no depth‐of‐field artifacts are present; and because this is a modification of the standard technique used by histology laboratories typically employed by medicolegal offices, no additional equipment or training is required.
Tech Support
Section titled “Tech Support”Original Source
Section titled “Original Source”References
Section titled “References”- 1956 - A comparative histological study of fossil and recent bones. Part 1
- 1966 - Studies of Haversian systems in man and some animals
- 2005 - Distinguishing between human and nonhuman bones: histometric method for forensic anthropology