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Voltammetric Determination of Ethionamide in Pharmaceutical Formulations and Human Urine using a Boron-Doped Diamond Electrode

MetadataDetails
Publication Date2015-01-01
JournalJournal of the Brazilian Chemical Society
AuthorsBruno Regis Lyrio Ferraz, Fernando Roberto FigueirĂȘdo Leite, Bruna Lima Farias Batista, AndrĂ©a R. Malagutti
InstitutionsUniversidade Federal dos Vales do Jequitinhonha e Mucuri
Citations19

This work reports a simple and low cost voltammetric approach comprising a boron-doped diamond electrode (BDDE) to determine the antibiotic ethionamide (ETO).Cyclic voltammetry studies revealed that ETO exhibits an irreversible reduction peak at -0.95 V and an irreversible oxidation peak at +1.4 V onto BDDE (vs.saturated Ag/AgCl reference electrode) in Britton-Robinson buffer solution (pH 5.0, 0.1 mol L -1 ).Different voltammetric scan rates (from 10 to 150 mV s -1 ) suggested that the reduction of ETO on the BDDE surface is a diffusion-controlled process.Square wave voltammetry (SWV) optimized conditions showed a linear response to ETO from 1.00 to 80.0 ”mol L -1 (R 2 = 0.998) with a limit of detection of 0.294 ”mol L -1 and limit of quantification of 0.980 ”mol L -1 .The developed square wave voltammetric method was successfully used in the determination of ETO in human urine and pharmaceutical formulation.The ETO quantification results in pharmaceutical tablets obtained by SWV-BDDE were comparable to those found by official analytical protocols.