Measuring Nanoscale Thermostability of Cell Membranes with Single Gold–Diamond Nanohybrids
At a Glance
Section titled “At a Glance”| Metadata | Details |
|---|---|
| Publication Date | 2017-01-31 |
| Journal | Angewandte Chemie International Edition |
| Authors | Pei‐Chang Tsai, Chandra Prakash Epperla, Jo‐Shan Huang, Oliver Y. Chén, Chih‐Che Wu |
| Institutions | National Chi Nan University, National Tsing Hua University |
| Citations | 90 |
Abstract
Section titled “Abstract”Abstract Much of the current understanding of thermal effects in biological systems is based on macroscopic measurements. There is little knowledge about the local thermostability or heat tolerance of subcellular components at the nanoscale. Herein, we show that gold nanorod-fluorescent nanodiamond (GNR‐FND) hybrids are useful as a combined nanoheater/nanothermometer in living cells. With the use of a 594 nm laser for both heating and probing, we measure the temperature changes by recording the spectral shifts of the zero‐phonon lines of negatively charged nitrogen‐vacancy centers in FNDs. The technique allows us to determine the rupture temperatures of individual membrane nanotubes in human embryonic kidney cells, as well as to generate high temperature gradients on the cell membrane for photoporation and optically controlled hyperthermia. Our results demonstrate a new paradigm for hyperthermia research and application.