Quantitative measurements of non-covalent interactions with diamond based magnetic imaging
At a Glance
Section titled āAt a Glanceā| Metadata | Details |
|---|---|
| Publication Date | 2018-07-30 |
| Journal | Applied Physics Letters |
| Authors | Metin Kayci, Sean Lourette, Tao Wang, Lykourgos Bougas, Dmitry Budker |
| Institutions | University of California, Berkeley, Helmholtz Institute Mainz |
| Citations | 5 |
Abstract
Section titled āAbstractāWe present a technique employing dielectrophoretic (DEP) manipulation of surface immobilized complexes integrated with a magnetic imaging platform based on nitrogen-vacancy (NV) centers in diamond for the quantitative measurements of non-covalent interactions. The interdigitated microelectrodes closely spaced to the functionalized surface of the diamond plate provide a wide range of applied DEP forces for noninvasive manipulation of various molecular interactions, while the NV layer under the surface reports the unbinding dynamics. Given that biological samples do not present significant magnetic background and do not screen magnetic fields, our approach has many advantages over the fluorescent tagging where the optical signal is subject to photo-bleaching, auto-fluorescence, and instabilities. The high sensitivity and spatial resolution provided by NV-based magnetic imaging make this technique a useful tool for biophysical applications.
Tech Support
Section titled āTech SupportāOriginal Source
Section titled āOriginal SourceāReferences
Section titled āReferencesā- 2008 - Force spectroscopy with optical and magnetic tweezers [Crossref]