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Voltammetry as the First Method for Direct Determination of a Novel Antagonist of A2A Adenosine Receptors

MetadataDetails
Publication Date2019-08-07
JournalElectroanalysis
AuthorsKatarzyna Tyszczuk‐Rotko, MaƂgorzata Sztanke, Agnieszka Sasal, Krzysztof Sztanke
InstitutionsMedical University of Lublin, Maria Curie-SkƂodowska University
Citations2

Abstract In this article, for the first time, the analytical method for determination of a novel antagonist of A 2A adenosine receptors (8‐(4‐methoxyphenyl)‐4‐oxo‐4,6,7,8‐tetrahydroimidazo[2,1‐ c ][1,2,4]triazine‐3‐carbohydrazide, namely IMT), which can be used as a drug for liver diseases, was presented. For this purpose a commercially available boron‐doped diamond electrode (BDDE) in combination with differential pulse voltammetry (DPV) was applied. It was found by cyclic voltammetry (CV) that IMT displays at BDDE, as a sensor, two well‐defined oxidation peaks at potentials of 0.81 and 1.18 V and one reduction peak at 1.1 V vs. Ag/AgCl in 0.1 mol L −1 acetate buffer (pH 4.5±0.1). The oxidation and reduction mechanism of IMT was proposed. The developed DPV method allowed the successful determination of IMT in the range of 0.05-50 ÎŒmol L −1 with detection limit equal to 0.0094 ÎŒmol L −1 and without any chemical modifications and electrochemical pretreatment of the electrode surface. The proposed procedure allows the determination of IMT in vitro directly from urine samples.