Photonic toolbox for fast real-time polymerase chain reaction
At a Glance
Section titled âAt a Glanceâ| Metadata | Details |
|---|---|
| Publication Date | 2020-06-16 |
| Journal | Laser Physics Letters |
| Authors | Christapher Vincent, A. A. Voronin, Kyle Sower, Vsevolod V. Belousov, Sokolov Av |
| Institutions | Federal Medical-Biological Agency, Russian Quantum Center |
| Citations | 6 |
Abstract
Section titled âAbstractâWe present an optical scientist vision for a photonic platform for real-time polymerase chain reaction (qPCR) and reverse-transcription qPCR (qRT-PCR) that integrates a rapid-cycle fiber-optic PCR chamber with feedback-controlled laser heating, online fluorometric detection of PCR products, and all-optical thermometry using an optical readout from color centers of diamond nanoparticles. We show that, with a careful optimization of laser-induced heating and heat-removal geometry, this approach can radically shorten the qPCR thermal cycle relative to standard PCR systems, paving the way toward credit-card-size testing kits for fast point-of-care infection diagnosis. Faster photonic qPCR/qRT-PCR will enable a rapid and highly specific early detection of infections, including new viruses, such as SARS-CoV-2 at very early stagesâin most cases, even before the onset of disease, providing much-needed lead time for early treatment and disease spread prevention.