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Rapid Nanomolar Detection of Guaiacol from its Precursors Using a Core‐shell Reversed‐phase Column Coupled with a Boron‐doped Diamond Electrode

MetadataDetails
Publication Date2020-10-23
JournalElectroanalysis
AuthorsPhyllis E. Hayes, Alyah Buzid, John H. T. Luong, Jeremy D. Glennon
InstitutionsUniversity College Cork, King Faisal University
Citations8

Abstract The electrooxidation mechanisms of guaiacol (2‐methoxyphenol), a food and beverage spoilage metabolite, and its precursors; vanillic acid (VA), vanillin, and ferulic acid (FA) were investigated by cyclic voltammetry (CV) with a boron‐doped diamond (BDD) electrode. Reversed‐phase liquid chromatography (RPLC) together with a BDD electrode poised at +1.6 V vs. Pd/H 2 , was optimized for their sensitive detection. The separation was achieved in 60 s with a core‐shell column (HALO C 18 ). The detection limits of these analytes ranged from 10-30 nM. The method was applicable for the analysis of guaiacol and its precursors from a popular commercial drink.