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Comparative Evaluation of Direct Smear and Culture Methods for Detection of Trichomonas gallinae Infection in Pigeon and Chicken of Assam

MetadataDetails
Publication Date2022-11-28
JournalZenodo (CERN European Organization for Nuclear Research)
AuthorsMunmi Saikia, Kanta Bhattacharjee, Prabhat Chandra Sarmah, Dilip Kumar Deka
InstitutionsAssam Agricultural University
Citations2

<p><strong>ABSTRACT: </strong>A study was carried out in order to compare the detection of <em>Trichomonas gallinae</em> by examination of smears of throat swab and culture of protozoa collected from both pigeon and chicken. In the present study, five culture media viz. Modified Diamond’s Media, Nutrient broth, Medium 199, Minimum Essential Medium (MEM) and Roswell Park Memorial Institute-1640 (RPMI-1640) were used for culture of <em>T. gallinae</em>. Microscopic examination of wet mount/ Giemsa stained smear and culture of the <em>T. gallinae</em> revealed the latter method to be superior to wet mount preparation or staining methods.</p>