Electrochemical detection of isatin using flow injection analysis with amperometric detection
At a Glance
Section titled āAt a Glanceā| Metadata | Details |
|---|---|
| Publication Date | 2017-04-01 |
| Journal | The FASEB Journal |
| Authors | Simon Sanchez, Romana JaroÅ”ovĆ”, Greg M. Swain |
| Institutions | Michigan State University, St. Maryās University, Texas |
Abstract
Section titled āAbstractāIsatin is a heterocyclic compound that has been shown to have a wide range of biological activities. Isatin is found endogenously in humans and rodents. In rat models, isatin concentrations have been shown to increase with stress in the heart, brain, blood plasma, and urinary samples. In the rat, highest concentrations are in the vas deferens and seminal vesicles with levels in the heart somewhat higher than in the brain. The highest concentrations in the brain are found in the hippocampus and cerebellum. Isatin is electrochemically active and can be detected with electrochemical techniques. Of these techniques, flow injection analysis is a versatile technique used for the determination of easily oxidizable or reducible analytes. The performance of a nitrogenāincorporated tetrahedral amorphous carbon electrode and a boronādoped diamond electrode was evaluated using flow injection analysis with amperometric detection. The boronādoped diamond electrode is known for its excellent properties such as a low stable background current, weak molecular absorption, and microstructural stability, but has a high deposition temperature required for growth, (600-800 Ā°C). Similarly, the taāC:N electrode has been shown to exhibit many of the same attractive properties of the boronādoped diamond electrode such as a low background current, microstructural stability, and weak molecular absorption with the advantage of a low deposition temperature near room temperature (25-100 Ā°C). The analytical detection figures of merit such as the response precision, sensitivity, linear dynamic range and limit of detection for isatin in a 0.1 M phosphate buffer (pH 7.2) were determined for both electrodes.