Optimization of diamond sensor for trace detection of SARS-CoV-2 N-protein using Au nanoparticles
At a Glance
Section titled āAt a Glanceā| Metadata | Details |
|---|---|
| Publication Date | 2023-08-21 |
| Journal | Applied Physics Letters |
| Authors | Qianwen Zhang, Huaxiong Wu, Yuxiang Du, Minghui Zhang, Bangqiang Xu |
| Institutions | Xiāan Jiaotong University, Shaanxi Provincial Peopleās Hospital |
| Citations | 3 |
Abstract
Section titled āAbstractāIn this study, Au nanoparticles were introduced to functionalize hydrogen-terminated diamond (H-diamond) to optimize surface modification and improve the performance of diamond sensor for trace detection of SARS-CoV-2 N-protein. The Au nanoparticles were obtained by an electron beam evaporation system and annealing. Atom force microscope and scanning electron microscope were utilized to characterize the structure, morphology, and distribution of fabricated Au nanoparticles. The concentration of SARS-CoV-2 N-protein was determined by the investigation of transfer characteristics of a diamond solution gate field effect transistor. Sensitivity of this sensor was obtained as 27.43 mV/lg (N-protein concentration), which was higher than a regular H-diamond sensor. In addition, it had a wider linear detection range of 10ā15-10ā5 mg/ml and a lower limit of detection of 10ā15 mg/ml. Accordingly, the detection performance of diamond sensor has been greatly improved due to the functionalization of Au nanoparticles.
Tech Support
Section titled āTech SupportāOriginal Source
Section titled āOriginal SourceāReferences
Section titled āReferencesā- 2020 - Investigation of novel SARS-COV-2 variant. Variant of concern 202012/01 detection of an epidemiological cluster associated with a new variant of concern nomenclature of variants in the UK current epidemiological findings
- 2010 - Electrochemical detection of proteins using nanoparticles: Applications to diagnostics [Crossref]
- 2022 - Emergency SARS-CoV-2 variants of concern: Novel multiplex real-time RT-PCR assay for rapid detection and surveillance [Crossref]
- 2004 - Sensitive and specific monoclonal antibody-based capture enzyme immunoassay for detection of nucleocapsid antigen in sera from patients with severe acute respiratory syndrome [Crossref]
- 2005 - Double-antigen sandwich ELISA for detection of antibodies to SARS-associated coronavirus in human serum [Crossref]
- 2004 - SARS antibody test for serosurveillance [Crossref]
- 2022 - An overview on recent advances in biosensor technology and its future application [Crossref]
- 2021 - Ultrasensitive detection of SARS-CoV-2 antibody by graphene field-effect transistors [Crossref]
- 2022 - Selective and electronic detection of COVID-19 (Coronavirus) using carbon nanotube field effect transistor-based biosensor: A proof-of-concept study [Crossref]
- 2022 - Graphene oxide-graphene Van der Waals heterostructure transistor biosensor for SARS-CoV-2 protein detection [Crossref]