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6CCVD Research

Evaluate the Antimicrobial Properties of Double Antibiotic Paste (DAP) and Morinda Citrifolia and Propolis Paste (MCP) Used in Regenerative Endodontics - An In vitro Study

At a Glance

Section titled ā€œAt a Glanceā€
MetadataDetails
Publication Date2025-02-28
JournalJournal of Pharmacy And Bioallied Sciences
AuthorsBhavika A. Bhavsar, Vaishali Jain, Annu Kumari, P. K. Shukla, Riddhi Kirit Pandya
InstitutionsRKDF University, KIIT University

Abstract

Section titled ā€œAbstractā€

A BSTRACT Objective: To Evaluate The Antimicrobial Properties of Double Antibiotic Paste and Morinda citrifolia And Propolis Paste Used In Regenerative Endodontics. Materials and Method: Using intact human teeth, radicular dentin samples were prepared (dimensions 4Ɨ4Ɨ2 mm) by following a standard method. The samples were cut using a diamond saw at minimal speed using water as coolant and smoothed with abrasive papers. To expose the dentin tubules and eliminate the smear layer, they were treated with NaOCl (1.5%), distilled water, and EDTA (17%). A total of 30 dentin samples went through a three-week anaerobic infection with bacterial biofilms sourced from the root canal of an underdeveloped tooth showing pulp necrosis. Group 1 acted as the control, while Group 2 received treatment with DAP, and Group 3 was treated with a paste of Morinda citrifolia and Propolis. After a week, pastes were cleaned, and specimens were dipped in phosphate-buffered saline for a day. The biofilm was released from each specimen by sonicating and vortexing it for 30 seconds. It was then diluted, spiral-plated on blood agar, and cultured anaerobically for a day. An automated colony counter was utilized to measure colony-forming units (CFU/mL), and statistical analysis was conducted using the Wilcoxon rank-sum test. Result: The wash from dentin samples treated with DAP and MCP showed minimal CFU counts, indicating both pastesā€™ high efficiency against biofilm-forming microbes from the canals of an immature tooth exhibiting pulp necrosis. Conclusion: Dentin samples infused with DAP and MCP showed minimal CFU counts, indicating their high effectiveness against biofilm-forming microbes from the root canal of an underdeveloped tooth with pulp necrosis. Additional research is required to validate these findings.

Tech Support

Section titled ā€œTech Supportā€

Original Source

Section titled ā€œOriginal Sourceā€

References

Section titled ā€œReferencesā€
  1. 2008 - Regenerative potential of dental pulp
  2. 2012 - Direct effect of intracanal medicaments on survival of stem cells of the apical papilla [Crossref]
  3. 2012 - Molecular mechanisms underlying the therapeutic activities of propolis: A critical review
  4. 2008 - Evaluation of Morinda citrifolia as an endodontic irrigant [Crossref]
  5. 2014 - Overview on the current antibiotic containing agents used in endodontics [Crossref]
  6. 2011 - Regenerative endodontics: A state of the art [Crossref]
  7. 2015 - In vivo antimicrobial efficacy of 6% Morinda citrifolia, Azadirachta indica, and 3% sodium hypochlorite as root canal irrigants [Crossref]

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