Rapid measurement of plasma concentration of a molecular-targeted agent, pazopanib, with diamond sensor.
At a Glance
Section titled āAt a Glanceā| Metadata | Details |
|---|---|
| Publication Date | 2020-01-01 |
| Journal | Proceedings for Annual Meeting of The Japanese Pharmacological Society |
| Authors | Takuro Saiki, Genki Ogata, Rito Kato, Olga Razvina, Seishiro Sawamura |
| Institutions | Keio University, Niigata University |
Abstract
Section titled āAbstractāMolecular-targeted anticancer drugs elicit less toxicity than conventional reagents. Yet, patients often suffer from severe adverse effects. A reason is āfixed dosageā administration of the drug to all the patients regardless of their body size and complications; because of this strategy, the plasma concentration seems to exceed the therapeutic window occasionally. Although frequent measurement of the drug level at a clinical site is a solution, currently available methods such as mass spectrometry are time and cost consuming. To overcome these shortcomings, in this study, we developed a procedure with an electrochemical sensor composed of a conductive diamond, which yields more stable reactions than conventional materials. When guinea-pig plasma mixed with pazopanib, a multi-kinase inhibitor, was tested, the sensor detected a clinically relevant concentration of 3 to 300 ĀµM. Time and sample amount necessary for each series of the measurement was <1 min and 100 ĀµL, respectively. The sensor was repeatedly usable with minimal impairment of the sensitivity, saving the cost for the assay. This rapid and easily-handed method may enable therapeutic drug monitoring and accelerate tailored medicine for cancer.