Live cell quantum multiphysiology enabled by a manipulable single nanodiamond
At a Glance
Section titled āAt a Glanceā| Metadata | Details |
|---|---|
| Publication Date | 2025-04-01 |
| Journal | National Science Review |
| Authors | Yang Xu, Yibo Yang, Yuang Chen, Wenxin Zhu, Shaoyu Lyu |
| Institutions | First Affiliated Hospital Zhejiang University, Zhejiang University |
| Citations | 3 |
Abstract
Section titled āAbstractāABSTRACT The patch-clamp technique provides direct insight into electrophysiology. However, probing multiphysiology such as local temperature and electromagnetic field dynamics within a single live cell remains a challenge. Here we report live cell quantum multiphysiology using a manipulable-single-nanodiamond-based electron spin sensor. By electrically trapping and integrating a single nanodiamond onto a glass nanopipette, we achieve its 3D manipulation within a single live cell. This enables the first nanoscale controlled live cell quantum sensing of multiphysiology signals under native conditions, providing direct insight into localized intracellular activities. Our observations reveal a spatial heterogeneity in temperature over different sites within a single cell, indicative of local activity-induced heat production. Furthermore, temporally resolved relaxation measurements effectively capture the intracellular free-radical-mediated local electro-magnetic noise dynamics. We find a number of unexpected fluctuation phenomena of electromagnetic noise that may directly represent different types of intracellular free radical generation dynamics. Live cell quantum multiphysiology bridges the gap between high-physical-sensitivity-quantum sensing with high-spatiotemporal-resolution live cell measurements, opening up a new avenue for accessing cellular function with unprecedented capabilities.
Tech Support
Section titled āTech SupportāOriginal Source
Section titled āOriginal SourceāReferences
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