Trace Detection of Staphylococcal Enterotoxin B Based on Hydrogen-Terminated Diamond Solution-Gate Field-Effect Transistor
At a Glance
Section titled āAt a Glanceā| Metadata | Details |
|---|---|
| Publication Date | 2025-09-30 |
| Journal | Analytical Chemistry |
| Authors | Yuxiang Du, Qingyu Lv, Yongqiang Jiang, Wei Wang, Genqiang Chen |
| Institutions | Academy of Military Medical Sciences, Xiāan Jiaotong University |
Abstract
Section titled āAbstractāStaphylococcal enterotoxin B is the most hazardous of the various toxins produced by <i>Staphylococcus aureus</i>. Thus, trace detection of SEB is essential for prevention, diagnosis, and treatment. In this work, an electrochemical platform was constructed to detect SEB based on a hydrogen-terminated diamond solution-gate field-effect transistor. The connection of 1-pyrenebutyric acid-<i>N</i>-hydroxy succinimide ester and anti-SEB antibody on the sensing area was confirmed by atomic force microscopy, scanning electron microscopy, and X-ray photoelectron spectroscopy. Output and transfer characteristics of the device were investigated, which obtained a wide linear response from 10<sup>-15</sup> to 10<sup>-7</sup> g/mL, a high sensitivity of -49.37 mV/lg [SEB concentration], and a low detection limitation of 10<sup>-15</sup> g/mL.