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High-resolution multiphoton microscopy with a low-power continuous wave laser pump

MetadataDetails
Publication Date2018-02-06
JournalOptics Letters
AuthorsXiang-Dong Chen, Li Shen, Bo Du, Dong Yang, Ze-Hao Wang
InstitutionsUniversity of Science and Technology of China
Citations7

Multiphoton microscopy (MPM) has been widely used for three-dimensional biological imaging. Here, based on the photon-induced charge state conversion process, we demonstrated a low-power high-resolution MPM with a nitrogen vacancy (NV) center in diamond. Continuous wave green and orange lasers were used to pump and detect the two-photon charge state conversion, respectively. The power of the laser for multiphoton excitation was 40 μW. Both the axial and lateral resolutions were improved approximately 1.5 times compared with confocal microscopy. The results can be used to improve the resolution of the NV center-based quantum sensing and biological imaging.