Nitrogen-Vacancy Magnetic Relaxometry of Nanoclustered Cytochrome C Proteins
At a Glance
Section titled āAt a Glanceā| Metadata | Details |
|---|---|
| Publication Date | 2024-01-11 |
| Journal | Nano Letters |
| Authors | Suvechhya Lamichhane, Rupak Timalsina, Cody Schultz, Ilja Fescenko, Kapildeb Ambal |
| Institutions | University of Latvia, University of NebraskaāLincoln |
| Citations | 15 |
Abstract
Section titled āAbstractāNitrogen-vacancy (NV) magnetometry offers an alternative tool to detect paramagnetic centers in cells with a favorable combination of magnetic sensitivity and spatial resolution. Here, we employ NV magnetic relaxometry to detect cytochrome C (Cyt-C) nanoclusters. Cyt-C is a water-soluble protein that plays a vital role in the electron transport chain of mitochondria. Under ambient conditions, the heme group in Cyt-C remains in the Fe<sup>3+</sup> state, which is paramagnetic. We vary the concentration of Cyt-C from 6 to 54 Ī¼M and observe a reduction of the NV spin-lattice relaxation time (<i>T</i><sub>1</sub>) from 1.2 ms to 150 Ī¼s, which is attributed to the spin noise originating from the Fe<sup>3+</sup> spins. NV <i>T</i><sub>1</sub> imaging of Cyt-C drop-casted on a nanostructured diamond chip allows us to detect the relaxation rates from the adsorbed Fe<sup>3+</sup> within Cyt-C.